Summary: © 1997 Oxford University Press3508­3513 Nucleic Acids Research, 1997, Vol. 25, No. 17
Antisense oligonucleotide binding to U5 snRNP
induces a conformational change that exposes the
conserved loop of U5 snRNA
Gil Ast and Alan M. Weiner*
Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, PO Box 208114,
New Haven, CT 06520-8114, USA
Received April 18, 1997; Revised and Accepted July 7, 1997
ABSTRACT
Conformational rearrangements of the spliceosomal
small nuclear RNAs (U snRNAs) are essential for
proper assembly of the active site prior to the first
catalytic step of splicing. We have previously shown
that conformational changes caused by binding of an
antisense 2-O-methyl RNA oligonucleotide (BU5Ae) to
U5 snRNA nt 68­88 disrupted the U4/U5/U6 complex
and induced formation of the U1/U4/U5 and U2/U6
complexes. Here we show that the conformational
change induced by BU5Ae exposes the invariant loop
of U5 that binds the 5 exon and also reorganizes

Source: Ast, Gil - Department of Molecular Genetics and Biochemistry, Tel Aviv University

Collections: Biology and Medicine