Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
Electrode Implantation and SNI Surgery Male Sprague-Dawley rats (225-250 grams) were anesthetized using isofluorane 5%,
 

Summary: Electrode Implantation and SNI Surgery
Male Sprague-Dawley rats (225-250 grams) were anesthetized using isofluorane 5%,
and 30% N2O and 70% O2 and then implanted with eight channel microwire electrodes
(50mm diameter, NB Labs, Denton TX), in mPFC: 2.9 mm anterior, 1.0mm lateral, and
4.1mm ventral to Bregma. Rats were allowed to recover for two weeks then recorded for
baseline neuronal activity. Half the animals then underwent SNI (ligation of tibial and
peroneal branches of the sciatic, Decosterd and Woolf, 2000). Two weeks later the rats
were fear conditioned (as in Milad & Quirk, 2002 ) while recording from mPFC. Neuronal
activity was recorded using multiple channel amplifiers, the amplified signal from each
electrode was digitized (20kHz) and saved on a PC for off line spike sorting (Plexon Inc,
Dallas TX).
Fear Conditioning
Rats were pre-exposed to the conditioning cage for 20 min on the day prior to conditioning.
Next day, the rats were first adapted for 60 s and presented with a 30 s tone. Two minutes
later they received 5 pairings of a 30 sec tone that co-terminated with a 1 sec shock (0.8
mA) delivered to the stainless steel bar floor, each pairing separated by 2 min. The animals
were then returned to their home cage. Extinction trials were conducted 1 and 24 hours
later in which after a 2 min adaptation period the rats were presented with 10 x 30 sec tone
separated by 2 min. Electrophysiological recording were done only during extinction.
Data Analysis

  

Source: Apkarian, A. Vania - Department of Physiology, Northwestern University

 

Collections: Biology and Medicine