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The Neuropeptide PDF Acts Directly on Evening Pacemaker Neurons to Regulate Multiple Features of
 

Summary: The Neuropeptide PDF Acts Directly on Evening
Pacemaker Neurons to Regulate Multiple Features of
Circadian Behavior
Bridget C. Lear, Luoying Zhang, Ravi Allada*
Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, United States of America
Abstract
Discrete clusters of circadian clock neurons temporally organize daily behaviors such as sleep and wake. In Drosophila, a
network of just 150 neurons drives two peaks of timed activity in the morning and evening. A subset of these neurons
expresses the neuropeptide pigment dispersing factor (PDF), which is important for promoting morning behavior as well as
maintaining robust free-running rhythmicity in constant conditions. Yet, how PDF acts on downstream circuits to mediate
rhythmic behavior is unknown. Using circuit-directed rescue of PDF receptor mutants, we show that PDF targeting of just
,30 non-PDF evening circadian neurons is sufficient to drive morning behavior. This function is not accompanied by large
changes in core molecular oscillators in light-dark, indicating that PDF RECEPTOR likely regulates the output of these cells
under these conditions. We find that PDF also acts on this focused set of non-PDF neurons to regulate both evening activity
phase and period length, consistent with modest resetting effects on core oscillators. PDF likely acts on more distributed
pacemaker neuron targets, including the PDF neurons themselves, to regulate rhythmic strength. Here we reveal defining
features of the circuit-diagram for PDF peptide function in circadian behavior, revealing the direct neuronal targets of PDF
as well as its behavioral functions at those sites. These studies define a key direct output circuit sufficient for multiple PDF
dependent behaviors.
Citation: Lear BC, Zhang L, Allada R (2009) The Neuropeptide PDF Acts Directly on Evening Pacemaker Neurons to Regulate Multiple Features of Circadian

  

Source: Allada, Ravi - Department of Neurobiology and Physiology, Northwestern University

 

Collections: Biology and Medicine