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IHC PROTOCOL TITLE Date:______________________
 

Summary: _________________________________________________
IHC PROTOCOL TITLE
Date:______________________
Project Name, Slide or Animal numbers____________________________________________________________________
Goal:________________________________________________________________________________________________
Number & ID of
Slides:______________________________________________________________________________________
Air Dry/Deparaffinize Washing Buffer:__________________ Diluting Buffer:_1% BSA in __________________
Blocking:
---endogenous peroxidase: 0.03%H2O2 or 0.3% H2O2 in PBS for 30 min Date of purchase H2O2
followed by 3 washes with buffer
---endogenous biotin: 0.1%avidin 15 min followed by _____buffer rinses
0.01% biotin 15 min followed by _____buffer rinses
---endogenous collagen binding 1% BSA/PBS;--? add 10% serum _____followed by _____buffer rinses
Fixation for Frozens:
Acetone 10 min
o Immediate PBS washes
o Cover sections with 1% BSA/PBS or
10% NBF
Antigen Retrieval for Paraffins:

  

Source: Abagyan, Ruben - School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego

 

Collections: Biology and Medicine