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IEEE TRANSACTIONS ON CONTROL SYSTEMS TECHNOLOGY , VOL. XX, NO. XX. XXX 2009 1 Tracking nanometer-scale fluorescent particles
 

Summary: IEEE TRANSACTIONS ON CONTROL SYSTEMS TECHNOLOGY , VOL. XX, NO. XX. XXX 2009 1
Tracking nanometer-scale fluorescent particles
in two dimensions with a confocal microscope
Zhaolong Shen, Student Member, IEEE and Sean B. Andersson, Member, IEEE
Abstract
A system for tracking multiple nanometer-scale fluorescent particles in a confocal microscope and an experimental
validation is described. Position estimates of an individual fluorescent particle are generated from fluorescence intensity
measurements taken at a small number of discrete locations. Tracking is achieved by combining the estimation
procedure with a linear quadratic Gaussian (LQG) regulator. Multiple particles are tracked by combining the models
for individual particles into a single system, applying the same LQG framework, and then cycling the control through
each subsystem in turn. Experimental results are presented for single and multiple particles. For validation purposes,
during each experiment images from a charge-coupled device camera were captured and analyzed offline using a
standard Gaussian fit method. The estimated trajectories were in good agreement with those produced by the LQG
algorithm, thereby verifying the tracking scheme.
Index Terms
Fluorescence microscopy; Particle tracking; Linear-quadratic-Gaussian control
I. INTRODUCTION
The ability to image and analyze single molecules is a powerful tool in molecular biology that continues to
be used to significantly advance knowledge of a wide-range of systems [1]. Recent applications include the study
of the nuclear trafficking process of influenza virus infection by tracking single vRNPs in living cells in real

  

Source: Andersson, Sean B. - Department of Aerospace and Mechanical Engineering, Boston University

 

Collections: Engineering