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Immunological Response from an Entirely Carbohydrate Antigen. Design of Synthetic Vaccines Based on Tn-PS A1 Conjugates
 

Summary: S.I. 1
Immunological Response from an Entirely Carbohydrate Antigen. Design of Synthetic
Vaccines Based on Tn-PS A1 Conjugates
Ravindra A. De Silva, Qianli Wang, Tristan Chidley, Dananjaya K. Appulage, and Peter R. Andreana*
Wayne State University, Department of Chemistry, 5101 Cass Ave, Detroit, MI, 48202
*E-mail: pra@chem.wayne.edu
Supporting Information
Materials and Methods: All reagents were purchased from Aldrich unless otherwise mentioned. All
reactions were monitored using thin layer chromatography (TLC) on 0.25 mm Dynamic Adsorbents,
L.L.C. pre-coated silica gel (particle size 0.03-0.07 mm, catalog no. 84111, lot # LA2006). Column
chromatography was performed using Whatman Purasil 60 (230-400 mesh ASTM) silica gel.
Yields refer to chromatographically and spectroscopically pure compounds. Proton and carbon-13
NMR spectra were recorded using Varian Mercury 400, Varian 500 Direct Drive System
spectrometers and Bruker 700. The residual CDCl3 singlet at 7.26 ppm and residual triplet at 77
ppm were used as the standard for 1
H NMR and 13
C NMR spectra respectively. Signal patterns are
indicated as s, singlet; d, doublet; t, triplet; q, quartet; m, multiplet; dd, doublet of doublets; b, broad.
Mass spectra were recorded on a Micromass GCT at 70 eV. Bruker Daltonics TOF-300 MALDI
Ultraflex was used to calculate the moles of mercaptooxime bound to maleimide activated BSA. A

  

Source: Andreana, Peter R. - Department of Chemistry, Wayne State University

 

Collections: Chemistry