Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network

  Advanced Search  

Carbohydrate Research 337 (2002) 969976 www.elsevier.com/locate/carres

Summary: Carbohydrate Research 337 (2002) 969976
Efficient chemoenzymatic synthesis of globotriose and its
derivatives with a recombinant a-(14)-galactosyltransferase
Jianbo Zhang, Przemyslaw Kowal, Jianwen Fang, Peter Andreana,
Peng George Wang*
Department of Chemistry, Wayne State Uni6ersity, Detroit, MI 48202, USA
Received 18 January 2002; accepted 30 March 2002
A truncated a-(14)-galactosyltransferase (LgtC) gene from Neisseria meningitidis was cloned. The recombinant glycosyltrans-
ferase was expressed in Escherichia coli BL21 (DE3) strain with high specific activity (5 units/mg protein). Its acceptor specificity
was carefully characterized. Then the purified enzyme was utilized in highly efficient syntheses of globotriose and a variety of
a-(14)-galactosylated derivatives as potential antibacterial agents. 2002 Published by Elsevier Science Ltd.
Keywords: Globotriose; Chemoenzymatic synthesis; LgtC; a-(14)-Galactosyltransferase; Recombinant enzyme
1. Introduction
Carbohydrates are now recognized as being of major
importance in a great variety of physiological and
pathological processes.13
In fact, glycans adorning the
cells are involved in extensive recognition phenomena


Source: Andreana, Peter R. - Department of Chemistry, Wayne State University


Collections: Chemistry