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ORIGINAL ARTICLE An Expressed Sequence Tag Analysis of the Intertidal Brown

An Expressed Sequence Tag Analysis of the Intertidal Brown
Seaweeds Fucus serratus (L.) and F. vesiculosus (L.)
(Heterokontophyta, Phaeophyceae) in Response
to Abiotic Stressors
Gareth A. Pearson & Galice Hoarau & Asuncion Lago-Leston & James A. Coyer &
Michael Kube & Richard Reinhardt & Kolja Henckel & Ester T. A. Serrão &
Erwan Corre & Jeanine L. Olsen
Received: 9 February 2009 /Accepted: 6 May 2009 /Published online: 17 July 2009
# Springer Science + Business Media, LLC 2009
Abstract In order to aid gene discovery and uncover genes
responding to abiotic stressors in stress-tolerant brown
algae of the genus Fucus, expressed sequence tags (ESTs)
were studied in two species, Fucus serratus and Fucus
vesiculosus. Clustering of over 12,000 ESTs from three
libraries for heat shock/recovery and desiccation/rehydra-
tion resulted in identification of 2,503, 1,290, and 2,409
unigenes from heat-shocked F. serratus, desiccated
F. serratus, and desiccated F. vesiculosus, respectively.
Low overall annotation rates (18­31%) were strongly


Source: Alberto, Filipe - Centro de Ciencias do Mar, Universidade do Algarve
Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,


Collections: Biology and Medicine; Environmental Sciences and Ecology