Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
Mot1 Regulates the DNA Binding Activity of Free TATA-binding Protein in an ATP-dependent Manner*
 

Summary: Mot1 Regulates the DNA Binding Activity of Free TATA-binding
Protein in an ATP-dependent Manner*
Received for publication, November 10, 2002, and in revised form, January 10, 2003
Published, JBC Papers in Press, February 4, 2003, DOI 10.1074/jbc.M211445200
Russell P. Darst, Arindam Dasgupta, Chunming Zhu, Jer-Yuan Hsu§, Amy Vroom,
Tamara Muldrow, and David T. Auble¶
From the Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia 22908
Mot1 is an essential Snf2/Swi2-related Saccharomyces
cerevisiae protein that binds the TATA-binding protein
(TBP) and removes TBP from DNA using ATP hydroly-
sis. Mot1 functions in vivo both as a repressor and as an
activator of transcription. Mot1 catalysis of TBP DNA
disruption is consistent with its function as a repressor,
but the Mot1 mechanism of activation is unknown. To
better understand the physiologic role of Mot1 and its
enzymatic mechanism, MOT1 mutants were generated
and tested for activity in vitro and in vivo. The results
demonstrate a close correlation between the TBP DNA
disruption activity of Mot1 and its essential in vivo func-
tion. Previous results demonstrated a large overlap in

  

Source: Auble, David - Department of Biochemistry and Molecular Genetics, University of Virginia

 

Collections: Biology and Medicine