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Acetyl-coenzyme A synthetase 2 is a nuclear protein required for replicative longevity in Saccharomyces cerevisiae
 

Summary: Acetyl-coenzyme A synthetase 2 is a nuclear protein required
for replicative longevity in Saccharomyces cerevisiae
Alaric A. Falcon Shaoping Chen Michael S. Wood
John P. Aris
Received: 29 April 2009 / Accepted: 6 July 2009 / Published online: 19 July 2009
Springer Science+Business Media, LLC. 2009
Abstract Acs2p is one of two acetyl-coenzyme A syn-
thetases in Saccharomyces cerevisiae. We have prepared
and characterized a monoclonal antibody specific for
Acs2p and find that Acs2p is localized primarily to the
nucleus, including the nucleolus, with a minor amount in
the cytosol. We find that Acs2p is required for replicative
longevity: an acs2D strain has a reduced replicative life
span compared to wild-type and acs1D strains. Further-
more, replicatively aged acs2D cells contain elevated lev-
els of extrachromosomal rDNA circles, and silencing at the
rDNA locus is impaired in an acs2D strain. These findings
indicate that Acs2p-mediated synthesis of acetyl-CoA in
the nucleus functions to promote rDNA silencing and
replicative longevity in yeast.

  

Source: Aris, John P. - Department of Anatomy and Cell Biology, University of Florida

 

Collections: Biology and Medicine