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Molecular Biology Buffers And Solutions NOTE: Use ACS grade chemicals, and dH2O for all buffers.
 

Summary: Molecular Biology Buffers And Solutions
NOTE: Use ACS grade chemicals, and dH2O for all buffers.
Use Sigma Trizma Tris Base and Tris-HCl for all buffers.
10 X TE: 500 ml 1X Conc.
6.1 g Tris Base 10 mM
1.9 g Na2EDTA-2H2O 1 mM
Adjust to pH 8.0 with HCl.
Autoclave.
3M KOAc, pH 5.0: 500 ml
148 g KOAc
Add 100 ml dH2O and mix
Add 100 ml AcCOOH
Measure pH and add AcCOOH to pH 5.0 (About 200-250 ml of AcCOOH).
Add dH2O to about 450 ml.
Cool to room temperature and adjust pH to 5.0.
Bring to final volume with dH2O.
3M NaOAc, pH 5.0: 500 ml
204.1 g NaOAc-3H2O
Add 100 ml dH2O and mix
Add 100 ml AcCOOH

  

Source: Aris, John P. - Department of Anatomy and Cell Biology, University of Florida

 

Collections: Biology and Medicine