Summary: Phytologia (April 2008) 90(1) 83
OPTIMIZING PROCEDURES TO OBTAIN RELIABLE DNA
FINGERPRINTING DATA FOR USE IN SYSTEMATIC,
ECOLOGICAL AND EVOLUTIONARY STUDIES.
Robert P. Adams
Baylor University, Biology Department, P.O. Box 97388,
Waco, TX 76798, USA Robert_Adams@baylor.edu
Lori E. Baker
Baylor University, Anthropology Forensic Science and Archaeology
Department, P.O. Box 97388, Waco, TX 76798, USA
R. Naresh Pandey
Biology Department, Wright State University, 3640 Colonel Glenn
Highway, Dayton, OH 45435.
The use of PCR based fingerprinting using short primers is very
sensitive to variation in procedures. Strict laboratory procedures,
exceeding general molecular biology standards, must be followed.
Many laboratories have experienced difficulties in obtaining
reproducible banding. Using a stock solution greatly minimizes
pipetting small quantities, but the stability of a Taq stock is not well