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Plant Physiol. (1985) 77, 833-839 0032-0889/85/77/0833/07/$0 1.00/0
 

Summary: Plant Physiol. (1985) 77, 833-839
0032-0889/85/77/0833/07/$0 1.00/0
Nodule-Specifilc Polypeptides from Effective Alfalfa Root
Nodules and from Ineffective Nodules Lacking Nitrogenase'
Received for publication August 24, 1984 and in revised form December 4, 1984
NAOMI LANG-UNNASCH* AND FREDERICK M. AUSUBEL
Department ofGenetics, Harvard Medical School, Boston, Massachusetts 02115; and Department of
Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114
ABSTRACT
In addition to leghemoglobin, at least nine nodule-specific polypeptides
from the alfalfa (Medicago sativa L.)-Rhizobium meliloti symbiosis were
identified by immune assay. Some ofthese polypeptides may be subunits
oflarger proteins but none appeared to be subunits ofthe same multimeric
protein. All nine of the nodule-specific polypeptides were loalized to
within the plant cytosol; they were not found in extracts of bacteroids or
in the peribacteroid space. At least one of these nodule-specific polypep-
tides was found to be antigenically related to nodule-specific polypeptides
in pea and/or soybean. Ineffective nodules elicited by R. meliloti strains
containing mutations in four different genes required for nitrogenase
synthesis contained reduced concentrations of leghemoglobin and of

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine