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The EMBO Journal Vol.2 No. 10 pp. 1831 -1838, 1983 Construction of a correlated physical and genetic map of the
 

Summary: The EMBO Journal Vol.2 No. 10 pp. 1831 - 1838, 1983
Construction of a correlated physical and genetic map of the
Klebsiella pneumoniae hisDGO region using transposon Tn5
mutagenesis
F.J. de Bruijnl*, I.L. Stroke2, D.J. Marvel1 and
F.M. Ausubel1
Department of Cellular and Developmental Biology, Harvard University,
Cambridge, MA 02138, USA
Communicated by J. Schell
Received on 11 July 1983
Multicopy plasmids containing the hisDG region of Klebsiella
pneumoniae were mutagenized with transposon Tn5. The
resulting plasmids were examined for their ability to comple-
ment hisD and hisG mutations in Escherichia coli. The
physical location of Tn5 on each of the hisD::Tn5 and
hisG::Tn5 plasmids was determined by restriction endo-
nuclease analysis. By combining the two types of data, a
precise correlated physical and genetic map of the K. pneu-
moniae hisDG region was constructed. Based on this analysis,
the minimum sizes of the hisD and the hisG genes were

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine