Summary: Chemoenzymatic elaboration of monosaccharides
using engineered cytochrome P450BM3 demethylases
Jared C. Lewisa
, Sabine Bastiana
, Clay S. Bennettb,1
, Yu Fub
, Yuuichi Mitsudaa,2
, Mike M. Chena
, William A. Greenbergb
,
Chi-Huey Wongb,c
, and Frances H. Arnolda,3
aDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125; bDepartment of Chemistry, The Scripps Research
Institute, La Jolla, CA, 92037; and cGenomics Research Center, Academia Sinica, Nankang, Taipei 115, Taiwan
Contributed by Frances H. Arnold, August 10, 2009 (sent for review July 2, 2009)
Polysaccharides comprise an extremely important class of biopoly-
mers that play critical roles in a wide range of biological processes,
but the synthesis of these compounds is challenging because of
their complex structures. We have developed a chemoenzymatic
method for regioselective deprotection of monosaccharide sub-

Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology

Collections: Chemistry; Biology and Medicine