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745 J. Exp. Med. The Rockefeller University Press 0022-1007/97/02/745/09 $2.00 Volume 185, Number 4, February 17, 1997 745753
 

Summary: 745 J. Exp. Med. The Rockefeller University Press 0022-1007/97/02/745/09 $2.00
Volume 185, Number 4, February 17, 1997 745753
Mapping the Active Site of CD59
By JinghuaYu,* Ruben Abagyan, Shanghong Dong,*
Alexander Gilbert,*Victor Nussenzweig,* and Stephen Tomlinson*
From the *Department of Pathology, NewYork University Medical Center, NewYork 10016 and
Department of Biochemistry,The Skirball Institute, NewYork 10016
Summary
CD59 is a widely distributed membrane-bound inhibitor of the cytolytic membrane attack
complex (MAC) of complement. This small (77 amino acid) glycoprotein is a member of the
Ly6 superfamily of proteins and is important in protecting host cells from the lytic and proin-
flammatory activity of the MAC. CD59 functions by binding to C8 and/or C9 in the nascent
MAC and interfering with C9 membrane insertion and polymerization. We present data ob-
tained from a combination of molecular modeling and mutagenesis techniques, which together
indicate that the active site of CD59 is located in the vicinity of a hydrophobic groove on the
face of the molecule opposite to a "hydrophobic strip" suggested earlier. In addition, removal
of the single N-linked glycosylation site at Asn18 of CD59 resulted in an enhancement of
complement inhibitory activity.
Activation of complement results in the formation of
the proteolytic C3/C5 convertases, which can initiate

  

Source: Abagyan, Ruben - School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego

 

Collections: Biology and Medicine