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Directed evolution of a genetic circuit Yohei Yokobayashi*, Ron Weiss
 

Summary: Directed evolution of a genetic circuit
Yohei Yokobayashi*, Ron Weiss
, and Frances H. Arnold*
*Division of Chemistry and Chemical Engineering, California Institute of Technology, 210-41, 1200 East California Boulevard, Pasadena, CA 91125; and
Department of Electrical Engineering, Princeton University, B-312, E-Quad, Princeton, NJ 08544
Edited by Charles R. Cantor, Sequenom, Inc., San Diego, CA, and approved October 21, 2002 (received for review September 3, 2002)
The construction of artificial networks of transcriptional control
elements in living cells represents a new frontier for biological
engineering. However, biological circuit engineers will have to
confront their inability to predict the precise behavior of even the
most simple synthetic networks, a serious shortcoming and chal-
lenge for the design and construction of more sophisticated ge-
netic circuitry in the future. We propose a combined rational and
evolutionary design strategy for constructing genetic regulatory
circuits, an approach that allows the engineer to fine-tune the
biochemical parameters of the networks experimentally in vivo. By
applying directed evolution to genes comprising a simple genetic
circuit, we demonstrate that a nonfunctional circuit containing
improperly matched components can evolve rapidly into a func-
tional one. In the process, we generated a library of genetic devices

  

Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology
Batzoglou, Serafim - Department of Computer Science, Stanford University

 

Collections: Biology and Medicine; Biotechnology; Chemistry; Computer Technologies and Information Sciences