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Genome-wide bioinformatic and molecular analysis of introns in Saccharomyces cerevisiae
 

Summary: Genome-wide bioinformatic and molecular
analysis of introns in Saccharomyces cerevisiae
MARC SPINGOLA,1
LESLIE GRATE,2
DAVID HAUSSLER,2
and MANUEL ARES, JR.1
1
Center for the Molecular Biology of RNA, Sinsheimer Laboratories, University of California­Santa Cruz,
Santa Cruz, California 95064, USA
2
Department of Computer and Information Sciences, Applied Sciences Building, University of California­Santa Cruz,
Santa Cruz, California 95064, USA
ABSTRACT
Introns have typically been discovered in an ad hoc fashion: introns are found as a gene is characterized for other
reasons. As complete eukaryotic genome sequences become available, better methods for predicting RNA process-
ing signals in raw sequence will be necessary in order to discover genes and predict their expression. Here we
present a catalog of 228 yeast introns, arrived at through a combination of bioinformatic and molecular analysis.
Introns annotated in the Saccharomyces Genome Database (SGD) were evaluated, questionable introns were re-
moved after failing a test for splicing in vivo, and known introns absent from the SGD annotation were added. A novel
branchpoint sequence, AAUUAAC, was identified within an annotated intron that lacks a six-of-seven match to the

  

Source: Ares Jr., Manny - Department of Molecular, Cell, and Developmental Biology, University of California at Santa Cruz

 

Collections: Biology and Medicine