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A Three-Dimensional Structural Dissection of Drosophila Polytene Chromosomes

Summary: A Three-Dimensional Structural Dissection of Drosophila
Polytene Chromosomes
Yoji Urata, Susan J. Parmelee, David A. Agard, and John W. Sedat
Department of Biochemistry and Biophysics and The Howard Hughes Medical Institute, University of California, San Francisco,
California 94143-0554
Abstract. We have analyzed the three-dimensional
structural details of Drosophila melanogasterpolytene
chromosome bands and interbands using three-dimen-
sional light microscopy and a novel method of sample
preparation that does not involve flattening or stretch-
ing the chromosomes. Bands have been visualized in
unfixed chromosomes stained with the DNA specific
dye 4,6-Diamidino-2-phenylindole (DAPI). Interbands
have been visualized using fixed chromosomes that
have been immunostained with an antibody to RNA
polymerase II. Additionally, these structures have been
analyzed using in situ hybridization with probes from
specific genetic loci (Notch and white).
Bands are seen to be composed of ~36 substructural
features that measure 0.2-0.4 txm in diameter. We sug-


Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco


Collections: Biotechnology; Biology and Medicine