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Proc. Nati. Acad. Sci. USA Vol. 82, pp. 134-138, January 1985
 

Summary: Proc. Nati. Acad. Sci. USA
Vol. 82, pp. 134-138, January 1985
Developmental Biology
Multiple polymorphic a- and ,8-tubulin mRNAs are present in sea
urchin eggs
DESPINA ALEXANDRAKI* AND JOAN V. RUDERMAN
Department of Anatomy, Harvard Medical School, 25 Shattuck Street, Boston, MA 02115
Communicated by Roy J. Britten, August 20, 1984
ABSTRACT Multiple a- and -tubulin RNAs were found
in the mature unfertilized eggs of the sea urchin Lytechinus
pictus. The a-tubulin RNAs were polymorphic in number,
size, and relative amounts in the eggs ofdifferent females. Five
to seven different size classes [1.75-4.2 kilobases (kb)] were
detected on RNA gel blots. All egg preparations contained
variable amounts of 1.8- and 2.25-kb (-tubulin RNAs, and a
few of them contained an additional 2.9-kb (-tubulin RNA.
The total amount of a-tubulin RNA did not always parallel
that of 3-tubulin RNA. A portion of all of the various a- and
(-tubulin RNAs were polyadenylylated. RNase H digestions
ruled out the possibility that some ofthese RNAs represented a

  

Source: Alexandraki, Despina - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas

 

Collections: Biology and Medicine; Biotechnology