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Proc. Natl. Acad. Sci. USA Vol. 88, pp. 7061-7065, August 1991
 

Summary: Proc. Natl. Acad. Sci. USA
Vol. 88, pp. 7061-7065, August 1991
Genetics
Limited functional equivalence of phylogenetic variation in small
nuclear RNA: Yeast U2 RNA with altered branchpoint
complementarity inhibits splicing and produces a
dominant lethal phenotype
(spliceosome assembly/small nuclear ribonucleoproteins/RNA structure)
LOREN MIRAGLIA*, SCOTT SEIWERTt, A. HALLER IGEL, AND MANUEL ARES, JR.:
Sinsheimer Laboratories, University of California, Santa Cruz, Santa Cruz, CA 95064
Communicated by John Abelson, April 17, 1991
ABSTRACT U2 is a highly conserved small nuclear RNA
essential for pre-mRNA splicing in mammals and yeast and for
trans-splicing in trypanosomes. To test the function of variant
U2 RNA structures from different organisms, we conducted
phylogenetic exchanges of U2 domains. Replacing nucleotides
1-120 of yeast U2 with the corresponding region of human U2
generates a U2 RNA that is correctly folded and functions in
yeast. In contrast, replacement of the branchpoint interaction
region of yeast U2 with the corresponding region from trypan-

  

Source: Ares Jr., Manny - Department of Molecular, Cell, and Developmental Biology, University of California at Santa Cruz

 

Collections: Biology and Medicine