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Cellular/Molecular Nociceptor and Hair Cell Transducer Properties of TRPA1, a
 

Summary: Cellular/Molecular
Nociceptor and Hair Cell Transducer Properties of TRPA1, a
Channel for Pain and Hearing
Keiichi Nagata,1 Anne Duggan,1,4 Gagan Kumar,1 and Jaime GarciŽa-An~overos1,2,3,4
Departments of 1
Anesthesiology, 2
Physiology, and 3
Neurology, 4
Northwestern University Institute for Neuroscience, Northwestern University Feinberg
School of Medicine, Chicago, Illinois 60611
Mechanosensorychannelsofsensorycellsmediatethesensationsofhearing,touch,andsomeformsofpain.TheTRPA1(amemberofthe
TRPfamilyofionchannelproteins)channelisactivatedbypain-producingchemicals,anditsinhibitionimpairshaircellmechanotrans-
duction. As shown here and previously, TRPA1 is expressed by hair cells as well as by most nociceptors (small neurons of dorsal root,
trigeminal, and nodose ganglia) and localizes to their sensory terminals (mechanosensory stereocilia and peripheral free nerves, respec-
tively). Thus, TRPA1 channels are proposed to mediate transduction in both hair cells and nociceptors. Accordingly, we find that
heterologouslyexpressedTRPA1displaychannelbehaviorsexpectedforbothauditoryandnociceptivetransducers.First,TRPA1andthe
hair cell transducer share a unique set of pore properties not described for any other channel (block by gadolinium, amiloride, gentami-
cin, and ruthenium red, a ranging conductance of 100 pS that is reduced to 54% by calcium, permeating calcium-induced potentiation
followed by closure, and reopening by depolarization), supporting a direct role of TRPA1 as a pore-forming subunit of the hair cell
transducer. Second, TRPA1 channels inactivate in hyperpolarized cells but remain open in depolarized cells. This property provides a

  

Source: Alford, Simon - Department of Biological Sciences, University of Illinois at Chicago

 

Collections: Biology and Medicine