Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
Copyright 2000 by the Genetics Society of America Bacillus thuringiensis (Bt) Toxin Susceptibility and Isolation of Resistance Mutants
 

Summary: Copyright 2000 by the Genetics Society of America
Bacillus thuringiensis (Bt) Toxin Susceptibility and Isolation of Resistance Mutants
in the Nematode Caenorhabditis elegans
Lisa D. Marroquin,1,* Dino Elyassnia,1,* Joel S. Griffitts,* Jerald S. Feitelson
and Raffi V. Aroian*
*Department of Biology, University of California, San Diego, California 92093 and
Akkadix Corporation, La Jolla, California 92037
Manuscript received March 15, 2000
Accepted for publication April 24, 2000
ABSTRACT
The protein toxins produced by Bacillus thuringiensis (Bt) are the most widely used natural insecticides
in agriculture. Despite successful and extensive use of these toxins in transgenic crops, little is known
about toxicity and resistance pathways in target insects since these organisms are not ideal for molecular
genetic studies. To address this limitation and to investigate the potential use of these toxins to control
parasitic nematodes, we are studying Bt toxin action and resistance in Caenorhabditis elegans. We demonstrate
for the first time that a single Bt toxin can target a nematode. When fed Bt toxin, C. elegans hermaphrodites
undergo extensive damage to the gut, a decrease in fertility, and death, consistent with toxin effects in
insects. We have screened for and isolated 10 recessive mutants that resist the toxin's effects on the
intestine, on fertility, and on viability. These mutants define five genes, indicating that more components
are required for Bt toxicity than previously known. We find that a second, unrelated nematicidal Bt toxin

  

Source: Aroian, Raffi V. - Division of Biological Sciences, University of California at San Diego

 

Collections: Biology and Medicine