· Animals & surgery: Two sets of Wistar-Kyoto
rats were followed: Set 1 was euthanized one week
after the chronic pain induction, and Set 2 three
weeks after. Both sets had four groups: Control,
Sham, CCI, and SNI. Four rats per group.
· Cytokines expression: Brain tissue samples
from anesthetized rats were dissected using RNA
free protocol. RNA was extracted from the samples
using standard protocol and reverse transcribed
(using Superscript II RT kit, GIBCO/BRL), amplified
at least 3 times (Pitossi et al. J. Neuroscience Res.
48:287,1997). Expression is quantified as cycles of
amplification to reach gene expression plateau,
relative to the house-keeping gene.
·Mechanical allodynia: Thresholds were assessed
using Von Frey filaments at various points after the
S u p p o r t e d b yS u p p o r t e d b y NIH NINDS NS42660NIH NINDS NS42660