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PHYSIOLOGY AND BIOCHEMISTRY Knockout of a starch synthase gene OsSSIIIa/Flo5 causes
 

Summary: PHYSIOLOGY AND BIOCHEMISTRY
Knockout of a starch synthase gene OsSSIIIa/Flo5 causes
white-core floury endosperm in rice (Oryza sativa L.)
Nayeon Ryoo Chul Yu Cheon-Seok Park Moo-Yeol Baik
In Myoung Park Man-Ho Cho Seong Hee Bhoo Gynheung An
Tae-Ryong Hahn Jong-Seong Jeon
Received: 3 December 2006 / Revised: 12 January 2007 / Accepted: 15 January 2007 / Published online: 13 February 2007
Springer-Verlag 2007
Abstract To elucidate the role of SSIIIa during starch
synthesis in rice (Oryza sativa L.) endosperm, we
characterized null mutants of this gene, generated
by T-DNA insertions. Scanning electron microscope
(SEM) analysis revealed that the starch granules
in these mutants are smaller and rounder compared
with the wild type controls, and that the mutant
endosperm is characterized by a loosely packed central
portion exhibiting a floury-like phenotype. Hence, the
OsSSIIIa (Oryza sativa SSIIIa) mutations are referred
to as white-core floury endosperm 5-1 (flo5-1) and flo5-2.
Based upon their X-ray diffraction patterns, the crys-

  

Source: An, Gynheung - Department of Life Science, Pohang University of Science and Technology

 

Collections: Biotechnology; Biology and Medicine