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Research article TheJournalofClinicalInvestigation http://www.jci.org
 

Summary: Research article
TheJournalofClinicalInvestigation http://www.jci.org
ATP and purinergic receptor­dependent
membrane traffic in bladder umbrella cells
Edward C.Y. Wang,1,2 Jey-Myung Lee,1 Wily G. Ruiz,1
Elena M. Balestreire,1,2 Maximilian von Bodungen,1 Stacey Barrick,1
Debra A. Cockayne,3 Lori A. Birder,1 and Gerard Apodaca1,2
1Renal-Electrolyte Division and Laboratory of Epithelial Cell Biology and 2Department of Cell Biology and Physiology,
University of Pittsburgh, Pittsburgh, Pennsylvania, USA. 3Roche Palo Alto, Palo Alto, California, USA.
Theumbrellacellsthatlinethebladderaremechanosensitive,andbladderfillingincreasestheapicalsurface
areaofthesecells;however,theupstreamsignalsthatregulatethisprocessareunknown.Increasedpressure
stimulatedATPreleasefromtheisolateduroepitheliumofrabbitbladders,whichwasblockedbyinhibitorsof
vesiculartransport,connexinhemichannels,ABCproteinfamilymembers,andnucleosidetransporters.Pres-
sure-inducedincreasesinmembranecapacitance(ameasureofapicalplasmamembranesurfaceareawhere
1µF1cm2)wereinhibitedbytheserosal,butnotmucosal,additionofapyraseorthepurinergicreceptor
antagonistPPADS.Uponadditionofpurinergicreceptoragonists,increasedcapacitancewasobservedevenin
theabsenceofpressure.Moreover,knockoutmicelackingexpressionofP2X2and/orP2X3receptorsfailedto
showincreasesinapicalsurfaceareawhenexposedtohydrostaticpressure.Treatmentsthatpreventedrelease
ofCa2+fromintracellularstoresoractivationofPKAblockedATPS-stimulatedchangesincapacitance.These
resultsindicatethatincreasedhydrostaticpressurestimulatesreleaseofATPfromtheuroepitheliumandthat

  

Source: Apodaca, Gerard - Departments of Medicine & Cell Biology and Physiology, University of Pittsburgh

 

Collections: Biology and Medicine