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Combining Genomic Tools to Dissect Multifactorial Virulence in Pseudomonas

Summary: Combining Genomic Tools to Dissect
Multifactorial Virulence in Pseudomonas
Daniel G. Lee, Jonathan M. Urbach, Gang Wu, Nicole T. Liberati,
Rhonda L. Feinbaum, and Frederick M. Ausubel
1 Introduction
The growing number of sequenced bacterial genomes, including those of important
pathogenic isolates, has made a significant impact on the field of bacterial pathogen-
esis (Raskin et al., 2006). Combined with other technical advances in the laboratory,
this wealth of information has made possible the development and widespread adop-
tion of genomic tools for the study of infectious disease. This chapter focuses on the
sequencing and functional analysis of PA14, a clinical isolate of the ubiquitous envi-
ronmental bacterium and important opportunistic human pathogen, Pseudomonas
aeruginosa. By comparing PA14 to the sequence of the less virulent P. aerugi-
nosa isolate, PAO1, we have identified genomic sequences absent in one or the
other strain in order to examine the relationship between genomic content and
To assess the importance of strain-specific genes and virulence, we tested 20
diverse P. aeruginosa strains, including PA14 and PAO1, in a Caenorhabdidits ele-
gans pathogenesis model and observed a wide range of pathogenic potential; how-


Source: Ausubel, Frederick M. - Department of Genetics, Harvard University


Collections: Biology and Medicine