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ChemBioChem 2002, No. 8 WILEY-VCH-Verlag GmbH, 69451 Weinheim, Germany, 2002 1439-4227/02/03/08 $ 20.00+.50/0 781 Modification of Galactose Oxidase to
 

Summary: ChemBioChem 2002, No. 8 WILEY-VCH-Verlag GmbH, 69451 Weinheim, Germany, 2002 1439-4227/02/03/08 $ 20.00+.50/0 781
Modification of Galactose Oxidase to
Introduce Glucose 6-Oxidase Activity
Lianhong Sun,[a]
Thomas Bulter,[a]
Miguel Alcalde,[a]
Ioanna P. Petrounia,[b]
and Frances H. Arnold*[a]
KEYWORDS:
carbohydrates enzymes glucose oxidase molecular
evolution mutagenesis
The selective oxidation of the 6-hydroxy group of D-glucose to
introduce an aldehyde functionality is not catalyzed by known
oxidase enzymes. Selective functionalization at the glucose C-6
position in oligo- and polysaccharides is a synthetically useful
reaction that would greatly facilitate further chemical modifica-
tions for food, pharmaceutical, and materials applications. We
chose the fungal enzyme galactose oxidase (D-galactose: oxygen
6-oxidoreductase, EC 1.1.3.9) as a starting point to try to generate
a glucose 6-oxidase. A well-characterized copper-containing

  

Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology

 

Collections: Chemistry; Biology and Medicine