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hybrid 46-pole undulator (wavelength range of 0.3 to 1.8 ) were used simultaneously to further enhance the
 

Summary: hybrid 46-pole undulator (wavelength range of 0.3 to
1.8 ) were used simultaneously to further enhance the
x-ray intensity at the crystal. Single x-ray pulses were
isolated with a microsecond shutter train (14) from the
ESRF storage ring operating in single-bunch mode at a
machine current of 15 mA. Laue diffraction intensities
were collected on an image-intensified charge-coupled
device detector (Thompson; ESRF). To minimize sys-
tematic errors such as those related to x-ray absorption,
we collected a dark data set for each crystal in addition
to the photoexcited data sets. Data sets consist of 30
images taken at crystal angular settings separated by
2 through a range of 60. Each image is a composite
of 10 150-ps exposures accumulated on the detec-
tor before readout, to enhance the relatively weak
diffraction data collected in single-bunch mode. No
significant radiation damage was detected over the
course of data collection. Data were reduced and
scaled with the LaueView software package (28).
Laue reflections stimulated by several energies (mul-

  

Source: Avraham, Karen - Department of Human Genetics and Molecular Medicine, Tel Aviv University

 

Collections: Biology and Medicine