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Effect of polyethylene glycol on the liquidliquid phase transition in aqueous protein solutions

Summary: Effect of polyethylene glycol on the liquid­liquid
phase transition in aqueous protein solutions
Onofrio Annunziata, Neer Asherie, Aleksey Lomakin, Jayanti Pande, Olutayo Ogun, and George B. Benedek*
Department of Physics, Center for Materials Science and Engineering, and Materials Processing Center, Massachusetts Institute of Technology,
Cambridge, MA 02139-4307
Contributed by George B. Benedek, August 22, 2002
We have studied the effect of polyethylene glycol (PEG) on the
liquid­liquid phase separation (LLPS) of aqueous solutions of
bovine D-crystallin ( D), a protein in the eye lens. We observe that
the phase separation temperature increases with both PEG con-
centration and PEG molecular weight. PEG partitioning, which is
the difference between the PEG concentration in the two coexist-
ing phases, has been measured experimentally and observed to
increase with PEG molecular weight. The measurements of both
LLPS temperature and PEG partitioning in the ternary D-PEG-
water systems are used to successfully predict the location of the
liquid­liquid phase boundary of the binary D-water system. We
show that our LLPS measurements can be also used to estimate the
protein solubility as a function of the concentration of crystallizing
agents. Moreover, the slope of the tie-lines and the dependence of


Source: Annunziata, Onofrio - Department of Chemistry, Texas Christian University
Asherie, Neer - Departments of Physics & Biology, Yeshiva University
Benedek, George B. - Department of Physics, Massachusetts Institute of Technology (MIT)


Collections: Biology and Medicine; Chemistry; Physics