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Suppression of RICE TELOMERE BINDING PROTEIN1 Results in Severe and Gradual Developmental Defects Accompanied
 

Summary: Suppression of RICE TELOMERE BINDING PROTEIN1 Results
in Severe and Gradual Developmental Defects Accompanied
by Genome Instability in Rice
Jong-Pil Hong,a Mi Young Byun,a Dal-Hoe Koo,b Kyungsook An,c Jae-Wook Bang,b In Kwon Chung,a
Gynheung An,c and Woo Taek Kima,1
a Department of Biology, College of Science, Yonsei University, Seoul 120-749, Korea
b School of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-764, Korea
c National Research Laboratory of Plant Functional Genomics, Department of Life Science,
Pohang University of Science and Technology, Pohang 790-784, Korea
Although several potential telomere binding proteins have been identified in higher plants, their in vivo functions are still
unknown at the plant level. Both knockout and antisense mutants of RICE TELOMERE BINDING PROTEIN1 (RTBP1)
exhibited markedly longer telomeres relative to those of the wild type, indicating that the amount of functional RTBP1 is
inversely correlated with telomere length. rtbp1 plants displayed progressive and severe developmental abnormalities in
both germination and postgermination growth of vegetative organs over four generations (G1 to G4). Reproductive organ
formation, including panicles, stamens, and spikelets, was also gradually and severely impaired in G1 to G4 mutants. Up to
11.4, 17.2, and 26.7% of anaphases in G2, G3, and G4 mutant pollen mother cells, respectively, exhibited one or more
chromosomal fusions, and this progressively increasing aberrant morphology was correlated with an increased frequency
of anaphase bridges containing telomeric repeat DNA. Furthermore, 35S:anti-RTBP1 plants expressing lower levels of
RTBP1 mRNA exhibited developmental phenotypes intermediate between the wild type and mutants in all aspects
examined, including telomere length, vegetative and reproductive growth, and degree of genomic anomaly. These results

  

Source: An, Gynheung - Department of Life Science, Pohang University of Science and Technology

 

Collections: Biotechnology; Biology and Medicine