Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
Toxoplasma gondii Rab6 Mediates a Retrograde Pathway for Sorting of Constitutively Secreted Proteins to the Golgi Complex*
 

Summary: Toxoplasma gondii Rab6 Mediates a Retrograde Pathway for
Sorting of Constitutively Secreted Proteins to the Golgi Complex*
Received for publication, September 13, 2002, and in revised form, November 25, 2002
Published, JBC Papers in Press, December 4, 2002, DOI 10.1074/jbc.M209390200
Timothy T. Stedman, A. Ross Sussmann, and Keith A. Joiner
From the Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8022
Toxoplasma gondii relies on protein secretion from
specialized organelles for invasion of host cells and es-
tablishment of a parasitophorous vacuole. We identify
T. gondii Rab6 as a regulator of protein transport be-
tween post-Golgi dense granule organelles and the
Golgi. Toxoplasma Rab6 was localized to cisternal rims
of the late Golgi and trans-Golgi network, associated
transport vesicles, and microdomains of dense granule
and endosomal membranes. Overexpression of wild-
type Rab6 or GTP-activated Rab6(Q70L) rerouted solu-
ble dense granule secretory proteins to the Golgi and
endoplasmic reticulum and augmented the effect of
brefeldin A on Golgi resorption to the endoplasmic
reticulum. Parasites expressing a nucleotide-free

  

Source: Arnold, Jonathan - Nanoscale Science and Engineering Center & Department of Genetics, University of Georgia

 

Collections: Biotechnology