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Summary: Transferring a Biosynthetic Cycle into a Productive
Escherichia coli Strain: Large-Scale Synthesis of
Galactosides
Xi Chen, Jianbo Zhang, Przemek Kowal, Ziye Liu,
Peter R. Andreana, Yuquan Lu, and Peng George Wang*
Department of Chemistry, Wayne State UniVersity
Detroit, Michigan 48202
ReceiVed May 12, 2001
ReVised Manuscript ReceiVed July 13, 2001
Discoveries concerning the important roles of glycoconjugates
in a variety of biological processes have led to an increased
demand for efficient synthetic methodologies adaptable for their
large-scale production.1 Compared to chemical synthesis, an
enzymatic approach utilizing glycosyltransferases has been proven
more efficient in the production of complex carbohydrates.2
Despite the increasing availability of various glycosyltransferases,
the high cost and inadequate availability of sugar nucleotides
required by these enzymes have severely limited their applica-
tions.3
Since sugar nucleotides only serve as cofactors in the
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