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Summary: ISOLATION AND GENETIC CHARACTERIZATION OF A MUTATION
AFFECTING RIBOSOMAL RESISTANCE TO CYCLOHEXIMIDE
IN TETRAHYMENA
MANUEL ARES, JR.l AND PETER J. BRUNS2
Section of Botany, Genetics and Development, Cornell University,
Ithaca, New York 14853
Manuscript received February 3, 1978
Revised copy received May 24,1978
ABSTRACT
A dominant mutation at a new locus affecting resistance to cycloheximide
has been isolated by exploiting a synergistic relationship with a previously
known mutation for cycloheximideresistance in Tetrahymena. The new muta-
tion (ChxB) was induced in a line homozygous for ChzA and was recovered
from that background by a new technique termed interrupted genomic exclu-
sion. Segregation data from the interrupted genomic exclusion suggest that
ChxA and ChzB are separate, linked loci showing 30% recombination. Mini-
mal lethal doses of cycloheximide for the four possible combinations of the
wild-type and mutant alleles of these two genes are: wild type 6 pg/ml, ChzA
125pg/ml, ChzB 10pg/ml, ChxA-ChxB 175pg/ml.
HE biosynthesis and fate of various components of ribosomes in the ciliated
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