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Summary: Snf2/Swi2-related ATPase Mot1 drives
displacement of TATA-binding protein
by gripping DNA
Rebekka O Sprouse1
, Michael Brenowitz2
and David T Auble1,
*
1
Department of Biochemistry and Molecular Genetics, University of
Virginia Health System, Charlottesville, VA, USA and 2
Department of
Biochemistry, The Albert Einstein College of Medicine, Bronx, NY, USA
Mot1 is a conserved Snf2/Swi2-related transcriptional
regulator that uses ATP hydrolysis to displace TATA-
binding protein (TBP) from DNA. Several models of the
enzymatic mechanism have been proposed, including
Mot1-catalyzed distortion of TBP structure, competition
between Mot1 and DNA for the TBP DNA-binding surface,
and ATP-driven translocation of Mot1 along DNA. Here,
DNase I footprinting studies provide strong support for
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