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Behavioral/Systems/Cognitive Numerous GABAergic Afferents to Locus Ceruleus in the
 

Summary: Behavioral/Systems/Cognitive
Numerous GABAergic Afferents to Locus Ceruleus in the
Pericerulear Dendritic Zone: Possible Interneuronal Pool
Gary Aston-Jones,1 Yan Zhu,1 and J. Patrick Card2
1Department of Psychiatry, University of Pennsylvania, Philadelphia, Pennsylvania 19104, and 2Department of Neuroscience, University of Pittsburgh,
Pittsburgh, Pennsylvania 15260
MostnucleiintheCNSarecomposedofprincipalneuronsthatprojecttootherareasandinterneuronsthatservetointegrateinformation
amongafferents.Thenoradrenergicbrainnucleuslocusceruleus(LC)hasappearedtobeanexceptiontothisgeneralrule,becausetheLC
is composed almost entirely of noradrenergic principal neurons. Here, we report that numerous small neurons in the peri-LC region
become retrogradely labeled after focal injections of wheat germ agglutinin­apo (inactivated) horseradish peroxidase conjugated to
colloidal gold, or pseudorabies virus (PRV), into the nuclear core of the rat LC. A substantial number of these neurons were routinely
foundwithinthedendriticfieldoftheLC,intheareasurroundingthecompactcell-denseregionclassicallydefinedasLC.Doublelabeling
revealed that a large percentage of these cells stained for GABA. Ultrastructural analyses revealed axodendritic and axosomatic contacts
between PRV-labeled afferents and LC neurons labeled with tyrosine hydroxylase immunohistochemistry. In addition, PRV-labeled
neuronsoraxonswereimmunopositiveforGABAinultrastructurallocalizations.Analysisofthesynaptologyofimmunopositiveprofiles
demonstrated that these LC afferents in the peri-LC region receive several non-LC synaptic inputs. These results indicate that a popula-
tion of small GABAergic neurons in the peri-LC dendritic zone may provide interneuronal integration for LC noradrenergic neurons.
Key words: norepinephrine; locus ceruleus; GABA; ultrastructure; interneurons; pseudorabies virus
Introduction
The brain nucleus locus ceruleus (LC) is the largest source of

  

Source: Aston-Jones, Gary - Department of Neurosciences, Medical University of South Carolina

 

Collections: Biology and Medicine