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The EMBO Journal Vol.17 No.13 pp.37383746, 1998 Depletion of yeast RNase III blocks correct U2 3 end

Summary: The EMBO Journal Vol.17 No.13 pp.3738­3746, 1998
Depletion of yeast RNase III blocks correct U2 3 end
formation and results in polyadenylated but
functional U2 snRNA
Sherif Abou Elela1,2,3 and Manuel Ares,Jr1
1Center for the Molecular Biology of RNA, Biology Department,
Sinsheimer Laboratories, University of California at Santa Cruz,
Santa Cruz, CA 95064, USA and 2Department of Microbiology,
Faculty of Medicine, UniversiteŽ de Sherbrooke, Sherbrooke, QueŽbec,
Canada J1H 5N4
3Corresponding author
e-mail: sabou@courrier.usherb.ca
Yeast U2 snRNA is transcribed by RNA polymerase II
to generate a single non-polyadenylated transcript. A
temperature-sensitive yeast strain carrying a disrup-
tion in RNT1, the gene encoding a homolog of RNase
III, produces 3 -extended U2 that is polyadenylated.
The U2 3 -flanking region contains a putative stem­
loop that is recognized and cleaved at two sites by
recombinant GST­Rnt1 protein in vitro. Removal of


Source: Ares Jr., Manny - Department of Molecular, Cell, and Developmental Biology, University of California at Santa Cruz


Collections: Biology and Medicine