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An improved strategy for automated electron microscopic tomography
 

Summary: An improved strategy for automated electron
microscopic tomography
Qingxiong S. Zheng,a
Michael B. Braunfeld,a
John W. Sedat,b
and David A. Agarda,b,*
a
The Howard Hughes Medical Institute, University of California, San Francisco, CA 94143-2240, USA
b
The W.M. Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California,
San Francisco, CA 94143-2240, USA
Received 7 November 2003, and in revised form 10 February 2004
Available online 9 April 2004
Abstract
A prediction-based scheme is proposed and implemented for automated electron microscopic tomography. By assuming that the
sample follows a simple geometric rotation and that the optical system can be characterized in terms of an offset between the optical
and mechanical axes, it is found that the image movement in the x, y, and z directions due to stage tilt can be dynamically predicted
with desired accuracy (15 nm in xy position and 100 nm in focus). Thus, the microscope optical system (beam/image shift and focus)
can be automatically adjusted to compensate for the predicted image movement prior to taking the projected image at each tilt
angle. As a consequence, it is not necessary to either record additional images for tracking and focusing during the course of data

  

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco

 

Collections: Biotechnology; Biology and Medicine