Summary: nature biotechnology volume 27 number 1 january 2009 49
assemble a eubacterial genome from short DNA fragment reads without
the use of a reference genome--a process referred to as de novo assembly.
In contrast to the technology in the first report of de novo assembly last
less raw data, and is especially useful for sequencing the rapidly evolving
genomes of bacteria--which, from even closely related strains, may dif-
fer by many genes often associated with pathogenesis. The authors use
their method to sequence the genome of the plant pathogen Pseudomonas
accounts for ~10% of all infections acquired in United States' hospitals.
have been missed by existing assembly methods requiring a reference
genome. The technique opens the door for the large-scale sequencing of
many individual bacterial isolates,a capability essential for understanding
mechanisms of bacterial evolution and toxicity. (Genome Res., published
online, doi:10.1101/gr.083311.108, 17 November 2008) CM
Phosphatidylserine is actively maintained in the inner leaflet of the
membranes of healthy cells,but`flips'and becomes exposed on the cell's