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nature biotechnology volume 27 number 1 january 2009 49 assemble a eubacterial genome from short DNA fragment reads without
 

Summary: nature biotechnology volume 27 number 1 january 2009 49
assemble a eubacterial genome from short DNA fragment reads without
the use of a reference genome--a process referred to as de novo assembly.
In contrast to the technology in the first report of de novo assembly last
year,thetechnologydescribedinthecurrentpaperrequiresabouttentimes
less raw data, and is especially useful for sequencing the rapidly evolving
genomes of bacteria--which, from even closely related strains, may dif-
fer by many genes often associated with pathogenesis. The authors use
their method to sequence the genome of the plant pathogen Pseudomonas
syringae,arelativeof thehumanpathogenPseudomonasaeroginosa,which
accounts for ~10% of all infections acquired in United States' hospitals.
Theythenidentifyunique P.syringaecandidatevirulencegenesthatwould
have been missed by existing assembly methods requiring a reference
genome. The technique opens the door for the large-scale sequencing of
many individual bacterial isolates,a capability essential for understanding
mechanisms of bacterial evolution and toxicity. (Genome Res., published
online, doi:10.1101/gr.083311.108, 17 November 2008) CM
Inside-out antivirals
Phosphatidylserine is actively maintained in the inner leaflet of the
membranes of healthy cells,but`flips'and becomes exposed on the cell's

  

Source: Alon, Uri - Departments of Molecular Cell Biology & Physics of Complex Systems, Weizmann Institute of Science

 

Collections: Biology and Medicine