| | |
Summary: Regulation of TATA-binding protein dynamics
in living yeast cells
Rebekka O. Sprouse*, Tatiana S. Karpova
, Florian Mueller
, Arindam Dasgupta*
, James G. McNally
,
and David T. Auble*§
*Department of Biochemistry and Molecular Genetics, University of Virginia Health System, 1340 Jefferson Park Avenue, Charlottesville, VA 22908; and
Center for Cancer Research Core Fluorescence Imaging Facility, Laboratory of Receptor Biology and Gene Expression, National Cancer Institute,
National Institutes of Health, 41 Library Drive, Bethesda, MD 20892
Edited by Robert H. Singer, Albert Einstein College of Medicine, Bronx, NY, and accepted by the Editorial Board July 17, 2008 (received for review
February 26, 2008)
Although pathways for assembly of RNA polymerase (Pol) II tran-
scription preinitiation complexes (PICs) have been well established
in vitro, relatively little is known about the dynamic behavior of Pol
II general transcription factors in vivo. In vitro, a subset of Pol II
factors facilitates reinitiation by remaining very stably bound to
the promoter. This behavior contrasts markedly with the highly
dynamic behavior of RNA Pol I transcription complexes in vivo,
|
