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Economic Botany 56(2) pp. 154164. 2002 2002 by The New York Botanical Garden Press, Bronx, NY 10458-5126 U.S.A.
 

Summary: Economic Botany 56(2) pp. 154164. 2002
2002 by The New York Botanical Garden Press, Bronx, NY 10458-5126 U.S.A.
USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISMS
(AFLP) TO IDENTIFY BLACK COHOSH (ACTAEA RACEMOSA)1
NYREE J. C. ZEREGA, SCOTT MORI, CHARLOTTE LINDQVIST,
QUNYI ZHENG, AND TIMOTHY J. MOTLEY
Zerega, Nyree J. C., Scott Mori (New York Botanical Garden, Bronx, NY 10458, USA),
Charlotte Lindqvist (Norwegian Forest Research Institute N-1432 As, Norway), Qunyi Zheng
(Pure World Botanicals, Inc., South Hackensack, NJ, USA), and Timothy J. Motley (New York
Botanical Garden, Bronx, NY 10458, USA). USING AMPLIFIED FRAGMENT LENGTH POLYMOR-
PHISMS (AFLP) TO IDENTIFY BLACK COHOSH (ACTAEA RACEMOSA). Economic Botany 56(2):154
164, 2002. The rhizome of Actaea racemosa L., commonly called black cohosh, is a popular
botanical dietary supplement used to treat female health concerns. The rhizomes used in black
cohosh products are often collected from the wild. To ensure quality control, it is imperative
that plants be correctly identified. This paper examines the use of the DNA fingerprinting
technique, AFLP, as an analytical means of identifying A. racemosa from three other closely
related sympatric species. To this end, 262 AFLP markers were generated, and one unique
fingerprint was identified for A. racemosa, whereas two, six, and eight unique fingerprints were
identified for the closely related species A. pachypoda, A. cordifolia, and A. podocarpa, re-
spectively. Two commercial black cohosh products were also subjected to AFLP analysis and

  

Source: Ault, James R. - Chicago Botanic Garden

 

Collections: Biology and Medicine