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Molecular Partitioning during Host Cell Penetration by Toxoplasma gondii

Summary: Molecular Partitioning during Host Cell Penetration by
Toxoplasma gondii
Audra J. Charron and L. David Sibley
Department of Molecular Microbiology, Washington
University School of Medicine, St. Louis, MO, 63110, USA
Corresponding author: L. David Sibley,
During invasion by Toxoplasma gondii, host cell trans-
membrane proteins are excluded from the forming para-
sitophorous vacuole membrane (PVM) by the tight
apposition of host and parasite cellular membranes. Pre-
vious studies suggested that the basis for the selective
partitioning of membrane constituents may be a prefer-
ence for membrane microdomains, and this hypothesis
was herein tested. The partitioning of a diverse group of
molecular reporters for raft and nonraft membrane sub-
domains was monitored during parasite invasion by
time-lapse video or confocal microscopy. Unexpectedly,
both raft and nonraft lipid probes, as well as both raft and
nonraft cytosolic leaflet proteins, flowed unhindered past


Source: Arnold, Jonathan - Nanoscale Science and Engineering Center & Department of Genetics, University of Georgia


Collections: Biotechnology