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Insect Biochemistry and Molecular Biology 32 (2002) 979989 www.elsevier.com/locate/ibmb
 

Summary: Insect Biochemistry and Molecular Biology 32 (2002) 979989
www.elsevier.com/locate/ibmb
Cloning and functional expression of a fat body-specific chitinase
cDNA from the tsetse fly, Glossina morsitans morsitans
J. Yan a,b
, Q. Cheng a,b
, S. Narashimhan c
, C.-B. Li a,b
, S. Aksoy a,
a
Department of Epidemiology and Public Health, Section of Vector Biology, Yale University School of Medicine, New Haven, CT 06510, USA
b
Institute of Genetics, Fudan University, Shanghai, People's Republic of China
c
Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510, USA
Received 12 October 2001; received in revised form 30 November 2001; accepted 20 December 2001
Abstract
A chitinase cDNA, GChit1 was isolated from Glossina morsitans morsitans and shown to be specifically expressed in fat body
tissue. GChit1 is encoded by a 1.6 kb mRNA with a putative open reading frame (ORF) of 460 amino acids (predicted pI
7.5, m.w. 51kDa) that contains a signal peptide domain and two potential N-linked glycosylation sites. The ORF exhibits hom-

  

Source: Aksoy, Serap - School of Public Health, Yale University

 

Collections: Biology and Medicine