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Summary: Identification of the Intermediates
of in Vivo Oxidation of 1,4-Dioxane
by Monooxygenase-Containing
Bacteria
S H A I L Y M A H E N D R A , * ,
C H R I S T O P H E R J . P E T Z O L D , , §
E D W A R D E . B A I D O O , , §
J A Y D . K E A S L I N G , , § A N D
L I S A A L V A R E Z - C O H E N , |
Department of Civil and Environmental Engineering and
Department of Chemical Engineering, University of
California, Berkeley, California 94720, and Physical
Biosciences Division and Earth Sciences Division, Lawrence
Berkeley National Laboratory, Berkeley, California 94720
1,4-Dioxane is a probable human carcinogen and an
emerging water contaminant. Monooxygenase-expressing
bacteria have been shown to degrade dioxane via growth-
supporting as well as cometabolic mechanisms. In this study,
the intermediates of dioxane degradation by monooxyge-
nase-expressing bacteria were determined by triple
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