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Formation of Indigo by Recombinant Mammalian Cytochrome P4501
 

Summary: Formation of Indigo by Recombinant Mammalian
Cytochrome P4501
Elizabeth M. J. Gillam,*,2
Anna Marie A. Aguinaldo, Lisa M. Notley,* Donghak Kim,
Ralf G. Mundkowski, Alexander A. Volkov, Frances H. Arnold,* Pavel Soucek,§
James J. DeVoss,¶
and F. Peter Guengerich,2
*Department of Physiology and Pharmacology and ¶
Department of Chemistry, University of Queensland, Brisbane, Queensland,
Australia 4072; Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena,
California 91125; Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University
School of Medicine, Nashville, Tennessee 37232; and §National Institute of Public Health,
Center of Occupational Diseases, Srobarova 48, Praha 10, 10042, Czech Republic
Received October 5, 1999
The development of bicistronic systems for coexpres-
sion of recombinant human cytochrome P450 enzymes
(P450s) with their redox partner, NADPH-cytochrome
P450 reductase (NPR), has enabled P450 activity to be
reconstituted within bacterial cells. During expression
of recombinant P450 2E1 and some other forms, we ob-

  

Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology

 

Collections: Chemistry; Biology and Medicine