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GPC-DMF Manual Krystyna Brzezinska (kbrzez@mrl.ucsb.edu)
 

Summary: GPC-DMF Manual
Krystyna Brzezinska (kbrzez@mrl.ucsb.edu)
May 2008
SAMPLE PREPARATION: Polymer must be perfectly soluble in DMF. Prepare solution 6-8 mg of the
polymer in 2 mL of DMF. Sample concentration affect both viscosity and injection volume. While small
sample amounts produce narrower peaks, viscous samples may require larger, more dilute solution.
Table 1. Recommended Sample Concentrations
Molecular-weight Range Sample Concentration
0 to 25,000 <0.25%
25,000 to 200,000 <0.1%
200,000 to 2,000,000 <0.05%
After filtration through 0.45 Ám filter transfer solution into the auto-sampler vial (3/4 of the vial must be
filled). Available small inserts for vials (150 and 300 ÁL).
ADD SAMPLES to the running GPC (only red icon is active):
1. Edit- Alter Running Sample- OK. Red and green icons should be active.
2. Insert rows, change name, vial #. Don't forget to load carousel with vials.
3. Don't change rows: Condition Columns; Equilibrate; Purge Injector; Purge Detector; Ramp Flow
Down, or PDA lamp OFF; Injection volume 100 ÁL and # of injections 1.
4. Click on the GREEN ICON. Only red icon should be active. For automatic results use "Run and
Report", continue on Fault- Run.

  

Source: Akhmedov, Azer - Department of Mathematics, University of California at Santa Barbara

 

Collections: Mathematics