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Isolation and characterization of a rice cysteine protease gene, OsCP1, using T-DNA gene-trap system
 

Summary: Isolation and characterization of a rice cysteine protease gene, OsCP1,
using T-DNA gene-trap system
Sanghyun Lee1
, Ki-Hong Jung2
, Gynheung An2
and Yong-Yoon Chung1,
*
1
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea (*author for corre-
spondence; e-mail: ychung@korea.ac.kr); 2
Division of Molecular and Life Sciences, Pohang University of
Science and Technology (POSTECH), Pohang 790-784, Korea
Received 10 October 2003; accepted in revised form 20 April 2004
Key words: anther, cell death, cysteine protease, rice, tapetum, T-DNA insertional mutagenesis
Abstract
The T-DNA gene-trap system has been efficiently used to elucidate gene functions in plants. We report here
a functional analysis of a cysteine protease gene, OsCP1, isolated from a pool of T-DNA insertional rice.
GUS assay with the T-DNA tagged line indicated that the OsCP1 promoter was highly active in the rice
anther. Sequence analysis revealed that the deduced amino acid sequence of OsCP1 was homologous to
those of papain family cyteine proteases containing the highly conserved interspersed amino acid motif,

  

Source: An, Gynheung - Department of Life Science, Pohang University of Science and Technology

 

Collections: Biotechnology; Biology and Medicine