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Summary: Molecular Ecology Resources (2009) 9, 425428 doi: 10.1111/j.1755-0998.2008.02436.x
© 2009 The Authors
Journal compilation © 2009 Blackwell Publishing Ltd
Blackwell Publishing Ltd
PERMANENT GENETIC RESOURCES
Isolation of highly polymorphic microsatellite loci for a
species with a large genome size: sharp-ribbed salamander
(Pleurodeles waltl)
MIRJAM S. VAN DE VLIET*, ONNO E. DIEKMANN*, ESTER A. SERRÃO* and PEDRO BEJA
*CCMAR-CIMAR, F.C.M.A- University of Algarve, Gambelas, P-8005-139 Faro, Portugal, ERENA, Ordenamento e Gestão de
Recursos Naturais Lda, Rua Robalo Gouveia, 1-1A, 1900-392 Lisboa, Portugal, CIBIO, Centro de Investigação em Biodiversidade e
Recursos Genéticos, Campus Agrário de Vairão, 4485-66 Vairão, Portugal
Abstract
Fourteen highly polymorphic microsatellite markers were developed and characterized for
the sharp-ribbed salamander, Pleurodeles waltl. Isolating microsatellites with more than 12
single repeat type units was only successful for a tetranucleotide repeat (ATAG). Compared
to microsatellite libraries constructed simultaneously for two anuran amphibian species, a
greater number of primer pairs designed for P. waltl had to be discarded, due to consistent
amplification problems. Low amplification success rate for P. waltl may be due to its larger
genome size. Consequently, to avoid nonspecific binding and to increase amplification
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