Wade et al, "The Snf1 kinase and the proteasome-associated Rad23 regulate UV-
responsive gene expression."
Figure S1: SNF1-RAD23 genetic interaction is specific to UV irradiation.
(A) 10-fold serial dilutions of yeast cultures were spotted onto YPD, synthetic complete media
containing 2% glucose (SC) and synthetic media containing 2% raffinose (SR). There was no
synthetic growth defect of snf1 rad23 cells under these conditions.
(B) 10-fold serial dilutions of yeast cultures were spotted onto YPD containing the indicated
concentration of hydroxyurea (HU). Deletion of RAD23 did not enhance the HU sensitivity of
Wade et al.
Figure S2: Snf1 activation is important for UV resistance.
(A) UV survival curve of the indicated yeast strains. Results were obtained as in Figure 3A.
Unlike deletion of the catalytic subunit, deletion of SNF4, encoding the regulatory subunit of the
Snf1 kinase complex, had no effect on UV sensitivity of rad23 cells. This further supports the
idea that low level basal kinase activity is important for UV resistance whereas activation of
Snf1 kinase is not critical under these conditions.
(B) UV survival curve for the indicated strains; the experiment was performed as in Figure 3A.