Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
Identification of the Individual Residues That Determine Human CD59 Species Selective Activity*
 

Summary: Identification of the Individual Residues That Determine
Human CD59 Species Selective Activity*
(Received for publication, November 24, 1998, and in revised form, January 26, 1999)
Hui-fen Zhang, Jinghua Yu, Shaohua Chen, B. Paul Morgan, Ruben Abagyan§, and Stephen
Tomlinson¶
From the Department of Pathology, New York University Medical Center, §Department of Biochemistry, The Skirball
Institute, New York, New York 10016, and Department of Medical Biochemistry, University of Wales College of Medicine,
Heath Park, Cardiff, C4F 4XN United Kingdom
Formation of the cytolytic membrane attack complex
of complement on host cells is inhibited by the mem-
brane-bound glycoprotein, CD59. The inhibitory activity
of CD59 is species restricted, and human CD59 is not
effective against rat complement. Previous functional
analysis of chimeric human/rat CD59 proteins indicated
that the residues responsible for the species selective
function of human CD59 map to a region contained be-
tween positions 40 and 66 in the primary structure. By
comparative analysis of rat and human CD59 models
and by mutational analysis of candidate residues, we
now identify the individual residues within the 40­66

  

Source: Abagyan, Ruben - School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego

 

Collections: Biology and Medicine